New to MyHealth?
Manage Your Care From Anywhere.
Access your health information from any device with MyHealth. You can message your clinic, view lab results, schedule an appointment, and pay your bill.
ALREADY HAVE AN ACCESS CODE?
DON'T HAVE AN ACCESS CODE?
NEED MORE DETAILS?
MyHealth for Mobile
A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells
A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells ACTA BIOMATERIALIA Hertz, J., Robinson, R., Valenzuela, D. A., Layik, E. B., Goldberg, J. L. 2013; 9 (8): 7622-7629Abstract
The central nervous system consists of complex groups of individual cells that receive electrical, chemical and physical signals from their local environment. Standard in vitro cell culture methods rely on two-dimensional (2-D) substrates that poorly simulate in vivo neural architecture. Neural cells grown in three-dimensional (3-D) culture systems may provide an opportunity to study more accurate representations of the in vivo environment than 2-D cultures. Furthermore, each specific type of neuron depends on discrete compositions and physical properties of their local environment. Previously, we developed a library of hydrogels composed of poly(ethylene glycol) and poly(l-lysine) which exhibit a wide range of mechanical properties. Here, we identified specific scaffolds from this library that readily support the survival, migration and neurite outgrowth of purified retinal ganglion cells and amacrine cells. These data address important biological questions about the interaction of neurons with the physical and chemical properties of their local environment and provide further insight for engineering neural tissue for cell-replacement therapies following injury.
View details for DOI 10.1016/j.actbio.2013.04.048
View details for Web of Science ID 000322207700002
View details for PubMedID 23648573
View details for PubMedCentralID PMC3722500