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Methods for in vitro generation of human type 1 regulatory T cells.
Methods for in vitro generation of human type 1 regulatory T cells. Methods in molecular biology (Clifton, N.J.) Gregori, S., Roncarolo, M. G., Bacchetta, R. 2011; 677: 31-46Abstract
Type 1 regulatory T (Tr1) cells are adaptive regulatory T cells that are induced in the periphery upon chronic exposure to antigen (Ag) in a tolerogenic environment containing interleukin (IL)-10. Tr1 cells are Ag-specific; they produce high levels of IL-10 and TGF-ß in the absence of IL-4 and suppress T-cell responses via a cytokine-dependent mechanism. During the last decade, several protocols have been developed to generate Tr1 cell lines in vitro. In this chapter, we outline protocols to generate non-Ag- and Ag-specific Tr1 cell lines and assays used to characterize Tr1 cell phenotype and functions.
View details for DOI 10.1007/978-1-60761-869-0_3
View details for PubMedID 20941601